Our lab works on a range of projects related to the control of pre-mRNA splicing and its role in gene regulation. We study the mechanisms of action of splicing regulators, as well as how these proteins affect biological processes such as neuronal development, tumor progression, and the inflammatory response. Long-standing projects have examined two families of RNA binding proteins: the polypyrimidine tract binding proteins and the Rbfox proteins. Biochemical methods and in vitro systems are used to examine the molecular interactions of these proteins and understand how they can alter spliceosome assembly. Biological studies use genetic and genomic methods in cell culture models and conditional mutant mice to understand how these proteins, and their particular splicing targets, affect development and cellular phenotype.